Freeze

This documentation pertains to Freeze 2023_May of the Roche Elecsys NTK - Gothenburg dataset.


Origin

These cerebrospinal fluid assay results come from the laboratory of Henrik Zetterberg and Kaj Blennow.

Sample selection:

Samples were included from observations at which at least one aliquot of CSF was available that had been stored in a Fisher 1.5 mL flip top tube. 
This dataset includes 1058 observations on 652 participants (409 ADRC; 405 female; 43 URG; 52 MCI and 50 Dementia at first visit; 50 MCI and 51 Dementia at last visit; mean first and last ages of 62.9 and 64.4, respectively). Per data sharing agreements with the Oneida nation, data from Native American participants are not included in this dataset.

Analytes:

• Amyloid beta 40
• Amyloid beta 42
• Phosphorylated tau 181
• Total tau
• Neurogranin
• Neurofilament light-chain
• Alpha synuclein
• Glial fibrillary acidic protein
• sTREM2
• S100
• YKL40
• Interleukin-6

Derived Variables:

• Ab42/Ab40
• ptau181/Ab42
• ttau/Ab42
• 1/Ab42
• Ab42/Ab40 binary
• ptau181/Ab42 binary

Detection Limits:

• Ab42: 200-1700 pg/mL
• ptau181: 8-120 pg/mL
• ttau: LLOQ 80 pg/mL
• IL-6: LLOQ 1.5 pg/mL

Methods

A first paper describing the use of these markers in the Wisconsin cohorts is here:

Van Hulle C, Jonaitis EM, Betthauser TJ, et al. An examination of a novel multipanel of CSF biomarkers in the Alzheimer’s disease clinical and pathological continuum. Alzheimers Dement. 2021;17(3):431-445. https://doi.org/doi:10.1002/alz.12204

Data Dictionary

Funding / Acknowledgments

Papers including data obtained from these assays should acknowledge NIH R01 AG027161 (the Wisconsin Registry for Alzheimer's Prevention) and/or NIH P30 AG062715 (the Wisconsin Alzheimer's Disease Research Center).

Papers with Wisconsin coauthors that include data from this set must submit abstracts, posters, talks and papers to Roche for pre-publication review.