Freeze

This documentation pertains to Freeze 2024_November of the Roche Elecsys NTK - Gothenburg dataset.


Origin

These cerebrospinal fluid assay results come from the laboratory of Henrik Zetterberg and Kaj Blennow.

Sample selection:

Samples were included from observations at which at least one aliquot of CSF was available that had been stored in a Fisher 1.5 mL flip top tube.

This dataset includes 1036 observations on 642 participants (400 ADRC; 242 WRAP; 398 female; 32 URG; 52 MCI and 49 Dementia at first visit; 50 MCI and 50 Dementia at last visit; 2 participant(s) are aged 90+, of those not: mean first and last ages of 63.0 and 64.4, respectively; mean first and last days since baseline of 1231 and 1776, respectively). Per data sharing agreements with the Oneida nation, data from Native American participants are not included in this dataset.

Analytes:

• Amyloid beta 40
• Amyloid beta 42
• Phosphorylated tau 181
• Total tau
• Neurogranin
• Neurofilament light-chain
• Alpha synuclein
• Glial fibrillary acidic protein
• sTREM2
• S100
• YKL40
• Interleukin-6

Derived Variables:

• Ab42/Ab40
• ptau181/Ab42
• ttau/Ab42
• 1/Ab42
• Ab42/Ab40 binary
• ptau181/Ab42 binary

Detection Limits:

• Ab42: 200-1700 pg/mL
• ptau181: 8-120 pg/mL
• ttau: LLOQ 80 pg/mL
• IL-6: LLOQ 1.5 pg/mL

Methods

A first paper describing the use of these markers in the Wisconsin cohorts is here:

Van Hulle C, Jonaitis EM, Betthauser TJ, et al. An examination of a novel multipanel of CSF biomarkers in the Alzheimer’s disease clinical and pathological continuum. Alzheimers Dement. 2021;17(3):431-445. https://doi.org/doi:10.1002/alz.12204

Data Dictionary

Funding / Acknowledgments

Papers including data obtained from these assays should acknowledge NIH R01 AG027161 (the Wisconsin Registry for Alzheimer's Prevention) and/or NIH P30 AG062715 (the Wisconsin Alzheimer's Disease Research Center).

Papers with Wisconsin coauthors that include data from this set must submit abstracts, posters, talks and papers to Roche for pre-publication review.