Supercritical Fluid/Ultra High Performance Liquid Chromatography (SFC/UHPLC) Hybrid System
Function
The 1260 Infinity II SFC/UHPLC Hybrid System allows you to perform supercritical fluid chromatography (SFC) and ultrahigh-performance liquid chromatography (UHPLC) on a single instrument. The system supports pressures up to 600 bar in SFC mode and up to 800 bar in UHPLC mode. Switching between the two modes is easy and can be fully automated.
Contact
Judith Simcox
jsimcox@wisc.edu
Location
Room 363A, Hector F. DeLuca Biochemistry Labs (433 Babcock Dr)
Oversight
All maintenance will be performed by the Simcox laboratory.
Training
The SFC/UPHLC may require several training levels dependent upon the user’s skill level and familiarity with chromatography. We have generated a training plan that will include an annual training session, online training, and the potential for scheduled trainings. The online materials will ensure that users are always able to access the appropriate protocols and resources, lowering the barrier of use, and ensuring a self-tailored experience.
Annual training for SFC/UHPLC will be offered the second week of January and will take place over two half days. These trainings will be a hands-on training session that will require attendees to go through the online resources before. Attendees will go through a live session performing an MTBE extraction to isolate lipid and metabolite fractions, set up a sample run to measure ketones, and will be taught the basics of data analysis. Advanced users that have prior experience with HPLC will be allowed to take a shortened version of the onboarding that includes setting up the run method, review of sample preparation methods, and data export.
Online training and resources will be hosted through the Simcox lab website through a shared biochemistry login and password. Several protocols will be offered on our website including sample preparation and run methods. We will host training videos of sample preparation, an introduction to setting up a run method, and screen capture videos of step by step data analysis. We will also provide the Github links to all R packages for data analysis and R markdowns, R tutorial videos for using the R packages will be posted on the Simcox lab website.
Scheduled training will be offered by the Simcox lab, in the laboratory, and will be advertised on the Simcox laboratory website. These will include the standard onboarding of the annual training and require the users to complete the online training before.