Bio-ARROW - SmartForm - Aerosol Containment

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Aerosol Generating Activities

When choosing more than one containment option for an activity, explain when the different forms of containment are used in the “Containment More Than One” section. The table below includes examples for activities when multiple containment options have been selected.

 

Activity 

Activity Other

Containment

Containment Other

Containment More Than One 

Surgery/tissue, organ, cell harvest/necropsy


Performed on lab bench, no containment used; BSC

 

Surgery, tissue, organ, cell harvest, and necropsies are performed in the BSC for mice administered virus.  Surgery for untreated mice may be performed on the lab bench.

Blending, sonicating, homogenizing, of electroporation

 

Performed on lab bench, no containment used; BSC 

 

RG1 microbes are handled on the lab bench.  All procedures with RG2 microbes, human materials, or biological toxins are performed in the BSC

Animal inoculations

 

BSC; Other

Performed outside of containment in animal procedure room

RG1 microbes are handled on the lab bench.  All activities associated with RG2 microbes, human materials, or biological toxins are handled in the BSC. Large animal inoculations performed in animal procedure room, not in a BSC.

Opening culture plates, ampoules, tubes

 

Performed on lab bench, no containment used; BSC

 

RG1 microbes are handled on the lab bench. All activities associated with RG2 microbes, human materials, or biological toxins are handled in the BSC.

Flaming inoculating needles, slides, or loops

 

Performed on lab bench, no containment used

Flaming is only performed with RG1 microbes, not in a BSC

 

Animal cage changes


Performed on lab bench, no containment used; BSC

 

Cage changes for mice that have been administered virus or human cells must be performed in the BSC. Cage changes for untreated transgenic mice may be conducted on the lab bench.

Vortexing

 

BSC; Performed on lab bench, closed containers

 

Vortexing is only performed with closed containers.

Pipetting 

 

Performed on lab bench, no containment used; BSC

 

RG1 microbes are pipetted on the lab bench. All pipetting of RG2 microbes, human materials (cell culture/tissues), or biological toxins are performed in the BSC


 

Biological Materials Outside of Containment

If any activity in the “Aerosol Generating Activities” section is performed without containment on the benchtop, it should be addressed in this section. A brief description of the work being done and the agents worked with is sufficient. For instance, “All culturing and plasmid prep of E. coli K12 will be performed outside of containment.”

 

Flow Cytometry Containment

For work performed at the UW Carbone Cancer Center flow cytometry laboratory, contact the staff to determine what type of containment is appropriate for the materials you are sorting. https://cancer.wisc.edu/research/resources/flow/ 

Still have questions? Call the Office of Biological Safety (OBS) at 608-263-2037. We are happy to help.


 




Keywords:flow cytometry containment, aerosol   Doc ID:43086
Owner:Tara S.Group:ARROW - Institutional Biosafety Committee
Created:2014-08-21 16:15 CDTUpdated:2021-06-23 09:45 CDT
Sites:ARROW - Institutional Biosafety Committee
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