Bio-ARROW - SmartForm - Aerosol Containment

Bio-ARROW help

Aerosol Generating Activities

When choosing more than one containment option for an activity, explain when the different forms of containment are used in the “Containment More Than One” section.  For example, “activities with E. coli K12 are performed on a lab bench with no containment used; work with human cells is performed in a BSC.”

Activity  Activity Other Containment Containment Other Containment More Than One 
Surgery/tissue, organ, cell harvest/necropsy
Performed on lab bench, no containment used; BSC   Surgery, tissue, organ, cell harvest, and necropsies are performed in the BSC for mice administered virus.  Surgery
Blending, sonicating, homogenizing, of electroporation   Performed on lab bench, no containment used; BSC    RG1 microbes are handled on the lab bench.  All procdures with RG2 microbes, human materials, and toxins are performed in the BSC
Animal inoculations   BSC   RG1 microbes are handled on the lab bench.  All activities associated with RG2 microbes, human materials, and toxins are handle din the BSC.
Opening culture plates, ampoules, tubes   Performed on lab bench, no containment used; BSC   RG1 microbes are handled on the lab bench. All activities associated with RG2 microbes, human materials, and toxins are handled in the BSC.
Flaming inoculating needles, slides, or loops   Performed on lab bench, no containment used Flaming is only performed with RG1 microbes, not in a BSC  
Animal cage changes
Performed on lab bench, no containment used   Cage changes for mice that have been administered virus or human cells must be performed in the BSC. Cage changes for untreated transgenic mice may be conducted on the lab bench.
Vortexing   BSC; Performed on lab bench, closed containers   Vortexing is only performed with closed containers.
Pipetting    Performed on lab bench, no containment used; BSC   RG1 microbes are pipetted on the lab bench. All pipetting of RG2 microbes, human materials (cell culture/tissues), and toxins are performed in the BSC

Biological Materials Outside of Containment


If any activity in the “Aerosol generating activities” section is performed without containment on the benchtop, it should be addressed in this section. A brief description of the work being done and the agents worked with is sufficient. For instance, “All culturing and plasmid prep of E. coli K12 will be performed outside of containment.”

Flow Cytometry Containment

For work performed at the UWCCC flow cytometry laboratory, http://www.uwhealth.org/uw-carbone-cancer-center/for-researchers/shared-resources/flow-cytometry/flow-cytometry-shared-resource/27331, contact the staff to determine what type of containment is appropriate for the materials you are sorting.

Still have questions? Call the Office of Biological Safety (OBS) at 608-263-2037. We are happy to help.

 




Keywords:flow cytometry containment, aerosol   Doc ID:43086
Owner:Tara S.Group:ARROW - Institutional Biosafety Committee
Created:2014-08-21 15:15 CSTUpdated:2020-09-08 10:26 CST
Sites:ARROW - Institutional Biosafety Committee
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