Bio-ARROW - SmartForm - Disinfection and Inactivation
• Disinfection and Inactivation-Animal
• Disinfection and Inactivation-Plant
• Disinfection and Inactivation-General
• Autoclave Testing
• Spill and Release Procedure
Disinfection and Inactivation-Animal
It is the PI’s responsibility to provide information regarding disinfection and disposal of materials, even if animal care staff perform the tasks. Please contact the individuals who disinfect/dispose of the materials to see what methods are generally used in the facility. Please verify with the vivarium manager regarding the disinfectants available in your approved animal space(s).
Please see the UW Institutional Biosafety Committee Policy UW-6102 for Animal Waste and Carcass Disposal located in the UW-Madison Policy Library. Bedding is not incinerated at UW-Madison unless special permission is granted.
Ethanol evaporates quickly, not allowing for sufficient contact time. Therefore, it is a recommendation to avoid using ethanol by itself for disinfection.
Disinfectant information may be found in the UW-Madison Researchers’ Biosafety Manual. https://ehs.wisc.edu/wp-content/uploads/sites/1408/2020/08/EHS-BIO-GUI-033-V01.pdf
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Example #1 |
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Mice administered human cells |
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Biosafety Level |
ABSL2 |
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Surfaces |
Peroxigard 0.5% Hydrogen Peroxide, 2-minute contact time |
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Surfaces Other |
No Value Entered |
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Surfaces More Than One |
No Value Entered |
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Surfaces Lab |
No Value Entered |
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Surfaces Lab Other |
No Value Entered |
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Surfaces Lab More Than One |
No Value Entered |
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Bedding/Wastes |
Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes |
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Bedding/Wastes Other |
No Value Entered |
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Bedding/Wastes More Than One |
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Carcasses |
Picked up for incineration by EH&S |
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Carcasses Other |
No Value Entered |
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Carcasses More Than One |
No Value Entered |
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Cages/Pens |
Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes |
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Cages/Pens Other |
No Value Entered |
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Cages/Pens More Than One |
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Example #2 |
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Animal(s) The Following Info Applies To |
Mouse (Transgenic), no biological materials administered |
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Biosafety Level |
ABSL1 |
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Surfaces |
Peroxigard 0.5% Hydrogen Peroxide, 2-minute contact time |
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Surfaces Other |
No Value Entered |
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Surfaces More Than One |
No Value Entered |
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Surfaces Lab |
1% bleach (1:100 dilution of household bleach containing 5.25%-6.15% sodium hypochlorite) until dry |
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Surfaces Lab Other |
No Value Entered |
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Surfaces Lab More Than One |
No Value Entered |
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Bedding/Wastes |
No special handling required |
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Bedding/Wastes Other |
No Value Entered |
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Bedding/Wastes More Than One |
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Carcasses |
Picked up for incineration by EH&S |
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Carcasses Other |
No Value Entered |
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Carcasses More Than One |
No Value Entered |
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Cages/Pens |
No special handling required |
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Cages/Pens Other |
No Value Entered |
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Cages/Pens More Than One |
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Example #3 |
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Animal(s) The Following Info Applies To |
Zebrafish (transgenic), no biological materials administered |
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Biosafety Level |
ABSL1 |
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Surfaces |
Other |
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Surfaces Other |
Fish tank surfaces will be disinfected with 1% bleach for 10 minutes then rinsed with water. |
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Surfaces More Than One |
No Value Entered |
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Surfaces Lab |
No Value Entered |
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Surfaces Lab Other |
No Value Entered |
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Surfaces Lab More Than One |
No Value Entered |
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Bedding/Wastes |
Other |
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Bedding/Wastes Other |
Fish tank water will be disinfected with 10% bleach for 20 minutes then rinsed with water. |
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Bedding/Wastes More Than One |
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Carcasses |
Picked up for incineration by EH&S |
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Carcasses Other |
No Value Entered |
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Carcasses More Than One |
No Value Entered |
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Cages/Pens |
Other |
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Cages/Pens Other |
Fish tank water will be disinfected with 10% bleach for 20 minutes then rinsed with water. |
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Cages/Pens More Than One |
Nets are disinfected with 1% bleach for 10 minutes then rinsed with water. |
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Example #4 |
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Animal(s) The Following Info Applies To |
Rats administered viral vectors |
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Biosafety Level |
ABSL2 |
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Surfaces |
1 % Virkon S for 10 minutes, |
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Surfaces Other |
No Value Entered |
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Surfaces More Than One |
No Value Entered |
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Surfaces Lab |
No Value Entered |
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Surfaces Lab Other |
No Value Entered |
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Surfaces Lab More Than One |
No Value Entered |
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Bedding/Wastes |
Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes |
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Bedding/Wastes Other |
No Value Entered |
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Bedding/Wastes More Than One |
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Carcasses |
Picked up for incineration by EH&S |
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Carcasses Other |
No Value Entered |
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Carcasses More Than One |
No Value Entered |
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Cages/Pens |
Other |
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Cages/Pens Other |
Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes |
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Cages/Pens More Than One |
No Value Entered |
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Example #5 |
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Animal(s) The Following Info Applies To |
Pig administered recombinant human cells |
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Biosafety Level |
ABSL2 |
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Surfaces |
Peroxigard 0.5% Hydrogen Peroxide, 2-minute contact time |
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Surfaces Other |
No Value Entered |
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Surfaces More Than One |
No Value Entered |
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Surfaces Lab |
No Value Entered |
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Surfaces Lab Other |
No Value Entered |
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Surfaces Lab More Than One |
No Value Entered |
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Bedding/Wastes |
Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes |
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Bedding/Wastes Other |
No Value Entered |
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Bedding/Wastes More Than One |
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Carcasses |
Picked up for incineration by EH&S |
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Carcasses Other |
No Value Entered |
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Carcasses More Than One |
No Value Entered |
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Cages/Pens |
Pens will be sprayed in place with 10% bleach (1:10 dilution of household bleach containing 5.25%-6.15% sodium hypochlorite) and allowed to dry. |
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Cages/Pens Other |
No Value Entered |
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Cages/Pens More Than One |
No Value Entered |
Disinfection and Inactivation-Plant
It is the PI’s responsibility to provide information regarding disinfection and disposal of materials, even if greenhouse staff perform the tasks. Please contact the individuals who disinfect/dispose of the materials to see what methods are generally used in the facility.
Ethanol evaporates quickly, not allowing for sufficient contact time. Therefore, it is a recommendation to avoid using ethanol by itself for disinfection.
Disinfectant information may be found in the UW-Madison Researchers’ Biosafety Manual. https://ehs.wisc.edu/wp-content/uploads/sites/1408/2020/08/EHS-BIO-GUI-033-V01.pdf
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Example |
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Plants |
Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes |
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Plants Other |
No Value Entered |
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Plants Explain |
No Value Entered |
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Soil |
Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes |
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Soil Other |
No Value Entered |
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Soil Explain |
No Value Entered |
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Pots |
Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes |
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Pots Other |
No Value Entered |
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Pots Explain |
No Value Entered |
Disinfection and Inactivation-General
Autoclaving materials containing bleach is not recommended as harmful chlorine gas may be released; therefore, if both are listed, please clarify when either treatment with bleach or by autoclaving is done, as applicable.
Ethanol evaporates quickly, not allowing for sufficient contact time. Therefore, it is a recommendation to avoid using ethanol by itself for disinfection.
Disinfectant information may be found in the UW-Madison Researchers’ Biosafety Manual. https://ehs.wisc.edu/wp-content/uploads/sites/1408/2020/08/EHS-BIO-GUI-033-V01.pdf
MERI (Madison Environmental Resourcing, Inc.) typically uses the process of microwave technology to inactivate waste. MERI is a UW-Madison contracted commercial vendor. Please contact the Regulated Waste Program Manager within UW-Madison Environment, Health and Safety for information on MERI bin locations, inactivation processes, and available services.
Sharps are items designed to cut or puncture skin and sharp items contaminated with human blood and body fluids. Sharps containers in BSL1 or BSL2 labs are not required to be autoclaved before disposing through MERI. BSL3 labs, however, are required to autoclave the sharps containers prior to disposal through MERI.
Hazardous glass and plastic includes items that can cut or scrape if disposed of in normal trash containers. (e.g., Pasteur pipettes, other pipettes ,pipette tips, uncontaminated slides, coverslips, broken glass, any fragile items). These items may require modified disposal procedures.
For information on sharps and laboratory glass disposal, please see our website: https://ehs.wisc.edu/disposal-services/sharps-disposal/
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Example |
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Surface |
1% bleach (1:100 dilution of household bleach containing 5.25%-6.15% sodium hypochlorite) until dry |
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Surface Other |
No Value Entered |
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Surface More Than One |
No Value Entered |
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Microbes, OTCC, rDNA materials |
10% bleach for a minimum of 20 minutes, Autoclave at a minimum of 121 Degrees C for a minimum of 45 minutes; MERI disposal |
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Microbes, OTCC, rDNA materials Other |
No Value Entered |
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Microbes, OTCC, rDNA materials More Than One |
Any of the methods are used |
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Sharps |
MERI disposal |
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Sharps Other |
No Value Entered |
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Sharps More Than One |
No Value Entered |
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Plasticware/glass |
Autoclave at a minimum of 121 Degrees C for a minimum of 45 minutes; MERI disposal |
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Plasticware/glass Other |
No Value Entered |
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Plasticware Glass More Than One |
Any of the methods are used |
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BioToxins |
20% bleach for a minimum of 30 minutes |
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BioToxins Other |
No Value Entered |
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Biological Toxins More Than One |
No Value Entered |
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Other |
No Value Entered |
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Other Material |
No Value Entered |
Autoclave Testing
For information on how to test an autoclave and autoclave safety, please see our website: https://ehs.wisc.edu/labs-research/biological-safety/autoclaves/.
Spill and Release Procedure
Biological spill guidance and spill procedure templates are available on the OBS website: https://ehs.wisc.edu/labs-research/biological-safety/biological-spill-protocols/.
If you would like to use these templates, complete the header (title) at the top of the page then create a customized protocol using the dropdowns provided within the template. In the header, the line above "Lab Biohazardous Spill Protocol for" should list the PI or laboratory name. The line below "Lab Biohazardous Spill Protocol for" should indicate when the procedure will be used (e.g., specify the biological materials, location(s), or other information that indicates when the spill protocol would be used). If the same procedure will be used for all locations, each room does not need to be listed in the header.
Guidance for completing the templates may be found at https://ehs.wisc.edu/labs-research/biological-safety/biological-spill-protocols/.
A transgenic release document example is available on the OBS website: Release of Transgenic Materials pdf
If you would like to use this template, please edit template as appropriate to specify procedures for biological materials handled in your laboratory (i.e., remove any release example(s) not applicable or include any additional procedures).
Still have questions? Call the Office of Biological Safety (OBS) at 608-263-2037. We are happy to help.