Nuropath/Neuromuscle Microscope Camera
Using cellSens for capturing images, counting and taking measurements
Table of Contents
- Launch Software and Start Preview
- Capture Image
- Ki67 Count
- Measurement (Poly-line)
- Adjust Scale Bar Settings
- Adjust Annotation Settings
Launch Software and Start Preview
- On your desktop, double-click on the "cellSens Dimension" icon to launch the software
- Near the top of the window, click the "Acquisition" tab
- Just below Acquisition, click on the correct objective and then click "OK"
- Set Exposure: Automatic
- Set Live/Movie: 1360x1024
- Set Snapshot/Process: 4080x3072 (Pixel shift)
- In the left column, click "Live" to start the camera
Capture Image
- Enter image annotation text in the "Notes" box
- Click "Snapshot" to capture an image
- Multiple images may be captured and will appear as tables above the preview window.
- If you have notations (scale bar, magnification), clock in the "Image" menu and choose "Burn In Info"
- At the warning, click "OK" -- your notations will now be saved with the image
- When you're ready to save an image, click the save icon
- Choose the destination folder and image type, name the file and click the "Save" button
Ki67 Count
- To open the counting window, go to View --> Tool Windows --> Object Counting
- In the Object Counting window, double-click "< Enter class name >".
- Choose a name and color for the class
- Click the "Count Objects" crosshairs to begin counting.
- Click in the preview window to mark points. Note that the border around the preview is the color of the item currently being counted
Measuring (Poly-line)
- To open the measurement window, go to View --> Tool Windows --> Measurement and ROI
- In the Measurement and ROI window, click the "Polyline" icon
- Click on the starting endpoint of the measurement line
- Click on additional points to draw the line
- Right-click on the final point to terminate the line and calculate the length
- To change the color or weight of the line, right click on the Polyline row in the Measurement and ROI window and choose "Change Color..." or "Change Link Thickness..."
- To change the unit of the measurement, click on the Polyline row in the Measurement and ROI window to highlight it
- Click on the "Properties" icon
- It the Options window, expand the Measurement and ROI section and click on "Results"
- Change the "Prefix of the unit" setting to the desired prefix (m for mm, u for um, etc)
Adjust Scale Bar Settings
- Click on Tools --> Options
- Expand the "Scale Bar" section and click on "Format"
- Set the color and font size of the scale bar text
- Set the color, thickness and share of the scale bar
- In the "Scale Bar" menu, click "Display"
- Set the length of the scale bar
- Set the orientation (horizontal or vertical) of the scale bar
- Set the position of the scale bar on the image
- Click "OK"
Adjust Annotation Settings
- Click on Tools --> Options
- Expand the "Info Stamp" section and click on "General"
- You can set color, size and position of the annotation (including magnification and label)
- Click "OK" to return to the live preview window