Bio-ARROW - SmartForm - Aerosol Containment
Aerosol Generating Activities
Aerosol Generating Activities
When choosing more than one containment option for an activity, explain when the different forms of containment are used in the “Containment More Than One” section.
Aerosol Generating Activities with Animals
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EXAMPLE #1 |
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Animal Name |
Laboratory rat |
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Animal Name Other |
No Value Entered |
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Materials Administered |
Lentivirus |
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Animal Biosafety Level |
ABSL2 |
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Inoculation Containment |
BSC |
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Other Inoculation Containment |
No Value Entered |
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Inoculation Containment More Than One |
No Value Entered |
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Cage Change Containment |
BSC |
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Other Cage Change Containment |
No Value Entered |
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Cage Change Containment More Than One |
No Value Entered |
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Surgery Containment |
No Value Entered |
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Other Surgery Containment |
No Value Entered |
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Surgery Containment More Than One |
No Value Entered |
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Tissue, Organ, Cell Harvest/Necropsy Containment |
BSC |
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Other Tissue, Organ, Cell Harvest/Necropsy Containment |
No Value Entered |
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Tissue, Organ, Cell Harvest/Necropsy Containment More Than One |
No Value Entered |
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Other Aerosol Generating Activities |
No Value Entered |
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Other Activity Containment |
No Value Entered |
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Other Containment Used |
No Value Entered |
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Other Activity Containment More Than One |
No Value Entered |
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EXAMPLE #2 |
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Animal Name |
Rhesus macaque |
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Animal Name Other |
No Value Entered |
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Materials Administered |
SHIV |
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Animal Biosafety Level |
ABSL2 |
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Inoculation Containment |
Performed in animal procedure space, floor, table, no containment used (larger animals) |
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Other Inoculation Containment |
No Value Entered |
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Inoculation Containment More Than One |
No Value Entered |
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Cage Change Containment |
Animals in pens, or open caging, no containment used |
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Other Cage Change Containment |
No Value Entered |
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Cage Change Containment More Than One |
No Value Entered |
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Surgery Containment |
No Value Entered |
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Other Surgery Containment |
No Value Entered |
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Surgery Containment More Than One |
No Value Entered |
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Tissue, Organ, Cell Harvest/Necropsy Containment |
Necropsy downdraft table |
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Other Tissue, Organ, Cell Harvest/Necropsy Containment |
No Value Entered |
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Tissue, Organ, Cell Harvest/Necropsy Containment More Than One |
No Value Entered |
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Other Aerosol Generating Activities |
No Value Entered |
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Other Activity Containment |
No Value Entered |
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Other Containment Used |
No Value Entered |
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Other Activity Containment More Than One |
No Value Entered |
Aerosol Generating Activities with Plants
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EXAMPLE #1 |
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Plant Name |
Arabidopsis |
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Materials Administered |
Agrobacterium tumefaciens |
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Plant Biosafety Level |
BSL1-P |
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Inoculation Containment |
Performed on lab bench, no containment used |
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Other Inoculation Containment |
No Value Entered |
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Inoculation Containment More Than One |
No Value Entered |
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Care Containment |
Performed on lab bench, no containment used |
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Other Care Containment |
No Value Entered |
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Care Containment More Than One |
No Value Entered |
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Plant Manipulation/Seed Harvest Containment |
Performed on lab bench, no containment used |
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Other Plant Manipulation/Seed Harvest Containment |
No Value Entered |
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Plant Manipulation/Seed Harvest Containment More Than One |
No Value Entered |
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Other Aerosol Generating Activities |
No Value Entered |
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Other Activity Containment |
No Value Entered |
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Other Containment Used |
No Value Entered |
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Other Activity Containment More Than One |
No Value Entered |
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EXAMPLE #2 |
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Plant Name |
Solanum lycospersicum |
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Materials Administered |
Salmonella |
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Plant Biosafety Level |
BSL2-P |
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Inoculation Containment |
BSC |
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Other Inoculation Containment |
No Value Entered |
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Inoculation Containment More Than One |
No Value Entered |
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Care Containment |
BSC |
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Other Care Containment |
No Value Entered |
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Care Containment More Than One |
No Value Entered |
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Plant Manipulation/Seed Harvest Containment |
BSC |
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Other Plant Manipulation/Seed Harvest Containment |
No Value Entered |
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Plant Manipulation/Seed Harvest Containment More Than One |
No Value Entered |
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Other Aerosol Generating Activities |
No Value Entered |
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Other Activity Containment |
No Value Entered |
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Other Containment Used |
No Value Entered |
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Other Activity Containment More Than One |
No Value Entered |
Aerosol Generating Activities-General
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EXAMPLES |
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Activity |
Activity Other |
Containment |
Containment Other |
Containment More Than One |
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Blending, sonicating, homogenizing, of electroporation |
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Performed on lab bench, no containment used; BSC |
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RG1 microbes are handled on the lab bench. All procedures with RG2 microbes, human materials, or biological toxins are performed in the BSC |
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Opening culture plates, ampoules, tubes |
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Performed on lab bench, no containment used; BSC |
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RG1 microbes are handled on the lab bench. All activities associated with RG2 microbes, human materials, or biological toxins are handled in the BSC. |
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Flaming inoculating needles, slides, or loops |
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Performed on lab bench, no containment used |
Flaming is only performed with RG1 microbes |
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Vortexing |
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BSC; Performed on lab bench, closed containers |
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Vortexing is only performed with closed containers. |
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Pipetting |
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Performed on lab bench, no containment used; BSC |
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RG1 microbes are pipetted on the lab bench. All pipetting of RG2 microbes, human materials (cell culture/tissues), or biological toxins are performed in the BSC |
Biological Materials Outside of Containment
If any activity in the “Aerosol Generating Activities” section is performed without containment on the benchtop, it should be addressed in this section. A brief description of the work being done and the agents worked with is sufficient. For instance, “All culturing and plasmid prep of E. coli K12 will be performed outside of containment.”
Flow Cytometry Containment
For work performed at the UW Carbone Cancer Center flow cytometry laboratory, contact the staff to determine what type of containment is appropriate for the materials you are sorting. https://cancer.wisc.edu/research/resources/flow/
Still have questions? Call the Office of Biological Safety (OBS) at 608-263-2037. We are happy to help you.