Bio-ARROW - SmartForm - Disinfection and Inactivation

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•             Disinfection and Inactivation-Animal    

•             Disinfection and Inactivation-Plant   

•             Disinfection and Inactivation-General    

•             Autoclave Testing

•             Spill and Release Procedure

Disinfection and Inactivation-Animal    

It is the PI’s responsibility to provide information regarding disinfection and disposal of materials, even if animal care staff perform the tasks. Please contact the individuals who disinfect/dispose of the materials to see what methods are generally used in the facility. Please verify with the vivarium manager regarding the disinfectants available in your approved animal space(s).

Please see the UW Policy 6090 for Animal Waste and Carcass Disposal located in the UW-Madison Policy Library https://policy.wisc.edu/library/UW-6090.  Bedding is not incinerated at UW-Madison unless special permission is granted.

Ethanol evaporates quickly, not allowing for sufficient contact time. Therefore, it is a recommendation to avoid using ethanol by itself for disinfection.

Disinfectant information may be found in the UW-Madison Researchers’ Biosafety Manual. https://ehs.wisc.edu/wp-content/uploads/sites/1408/2020/08/EHS-BIO-GUI-033-V01.pdf

 

Example #1

Animal(s) The Following Info Applies To

Mice administered human cells

Biosafety Level

ABSL2

Surfaces

Peroxigard 0.5% Hydrogen Peroxide, 2-minute contact time

Surfaces Other

No Value Entered  

Surfaces More Than One

No Value Entered  

Surfaces Lab

No Value Entered  

Surfaces Lab Other

No Value Entered  

Surfaces Lab More Than One

No Value Entered  

Bedding/Wastes

Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes

Bedding/Wastes Other

No Value Entered  

Bedding/Wastes More Than One


No Value Entered  

Carcasses

Picked up for incineration by EH&S

Carcasses Other

 No Value Entered  

Carcasses More Than One

  No Value Entered  

Cages/Pens

Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes

Cages/Pens Other

  No Value Entered  

Cages/Pens More Than One


No Value Entered  

 

Example #2

Animal(s) The Following Info Applies To

Mouse (Transgenic), no biological materials administered

Biosafety Level

ABSL1

Surfaces

Peroxigard 0.5% Hydrogen Peroxide, 2-minute contact time

Surfaces Other

No Value Entered  

Surfaces More Than One

No Value Entered  

Surfaces Lab

1% bleach (1:100 dilution of household bleach containing 5.25%-6.15% sodium hypochlorite) until dry  

Surfaces Lab Other

No Value Entered  

Surfaces Lab More Than One

No Value Entered  

Bedding/Wastes

No special handling required

Bedding/Wastes Other

No Value Entered  

Bedding/Wastes More Than One


No Value Entered  

Carcasses

Picked up for incineration by EH&S

Carcasses Other

 No Value Entered  

Carcasses More Than One

  No Value Entered  

Cages/Pens

No special handling required

Cages/Pens Other

  No Value Entered  

Cages/Pens More Than One


No Value Entered  

 

Example #3

Animal(s) The Following Info Applies To

Zebrafish (transgenic), no biological materials administered

Biosafety Level

ABSL1

Surfaces

Other

Surfaces Other

Fish tank surfaces will be disinfected with 1% bleach for 10 minutes then rinsed with water.

Surfaces More Than One

No Value Entered  

Surfaces Lab

No Value Entered  

Surfaces Lab Other

No Value Entered  

Surfaces Lab More Than One

No Value Entered  

Bedding/Wastes

Other

Bedding/Wastes Other

Fish tank water will be disinfected with 10% bleach for 20 minutes then rinsed with water.

Bedding/Wastes More Than One


No Value Entered  

Carcasses

Picked up for incineration by EH&S

Carcasses Other

 No Value Entered  

Carcasses More Than One

  No Value Entered  

Cages/Pens

Other

Cages/Pens Other

Fish tank water will be disinfected with 10% bleach for 20 minutes then rinsed with water.

Cages/Pens More Than One

Nets are disinfected with 1% bleach for 10 minutes then rinsed with water.

 

Example #4

Animal(s) The Following Info Applies To

Rats administered viral vectors

Biosafety Level

ABSL2

Surfaces

1 % Virkon S for 10 minutes,

Surfaces Other

No Value Entered  

Surfaces More Than One

No Value Entered  

Surfaces Lab

No Value Entered  

Surfaces Lab Other

No Value Entered  

Surfaces Lab More Than One

No Value Entered  

Bedding/Wastes

Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes

Bedding/Wastes Other

No Value Entered  

Bedding/Wastes More Than One


No Value Entered  

Carcasses

Picked up for incineration by EH&S

Carcasses Other

 No Value Entered  

Carcasses More Than One

  No Value Entered  

Cages/Pens

Other

Cages/Pens Other

Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes

Cages/Pens More Than One

 No Value Entered  

 

Example #5

Animal(s) The Following Info Applies To

Pig administered recombinant human cells

Biosafety Level

ABSL2

Surfaces

Peroxigard 0.5% Hydrogen Peroxide, 2-minute contact time  

Surfaces Other

No Value Entered  

Surfaces More Than One

No Value Entered  

Surfaces Lab

No Value Entered  

Surfaces Lab Other

No Value Entered  

Surfaces Lab More Than One

No Value Entered  

Bedding/Wastes

Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes

Bedding/Wastes Other

No Value Entered  

Bedding/Wastes More Than One


No Value Entered  

Carcasses

Picked up for incineration by EH&S

Carcasses Other

 No Value Entered  

Carcasses More Than One

  No Value Entered  

Cages/Pens

Pens will be sprayed in place with 10% bleach (1:10 dilution of household bleach containing 5.25%-6.15% sodium hypochlorite) and allowed to dry.

Cages/Pens Other

 No Value Entered  

Cages/Pens More Than One

 No Value Entered  

 

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Disinfection and Inactivation-Plant    

It is the PI’s responsibility to provide information regarding disinfection and disposal of materials, even if greenhouse staff perform the tasks. Please contact the individuals who disinfect/dispose of the materials to see what methods are generally used in the facility.

Ethanol evaporates quickly, not allowing for sufficient contact time. Therefore, it is a recommendation to avoid using ethanol by itself for disinfection.

Disinfectant information may be found in the UW-Madison Researchers’ Biosafety Manual. https://ehs.wisc.edu/wp-content/uploads/sites/1408/2020/08/EHS-BIO-GUI-033-V01.pdf

 

Example

Plants

Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes

Plants Other

No Value Entered  

Plants Explain

No Value Entered  

Soil

Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes

Soil Other

No Value Entered  

Soil Explain

No Value Entered  

Pots

Autoclave at a minimum of 121 Degrees C for a minimum of 60 minutes

Pots Other

No Value Entered  

Pots Explain

No Value Entered  

 

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Disinfection and Inactivation-General    

Autoclaving materials containing bleach is not recommended as harmful chlorine gas may be released; therefore, if both are listed, please clarify when either treatment with bleach or by autoclaving is done, as applicable.

Ethanol evaporates quickly, not allowing for sufficient contact time. Therefore, it is a recommendation to avoid using ethanol by itself for disinfection.

Disinfectant information may be found in the UW-Madison Researchers’ Biosafety Manual. https://ehs.wisc.edu/wp-content/uploads/sites/1408/2020/08/EHS-BIO-GUI-033-V01.pdf

MERI (Madison Environmental Resourcing, Inc.) typically uses the process of microwave technology to inactivate waste. MERI is a UW-Madison contracted commercial vendor. Please contact the Regulated Waste Program Manager within UW-Madison Environment, Health and Safety for information on MERI bin locations, inactivation processes, and available services. 

Sharps are items designed to cut or puncture skin and sharp items contaminated with human blood and body fluids. Sharps containers in BSL1 or BSL2 labs are not required to be autoclaved before disposing through MERI.  BSL3 labs, however, are required to autoclave the sharps containers prior to disposal through MERI.

Hazardous glass and plastic includes items that can cut or scrape if disposed of in normal trash containers. (e.g., Pasteur pipettes, other pipettes ,pipette tips,  uncontaminated slides, coverslips, broken glass, any fragile items). These items may require modified disposal procedures.

For information on sharps and laboratory glass disposal, please see our website:  
https://ehs.wisc.edu/disposal-services/sharps-disposal/ 

Example

Surface

1% bleach (1:100 dilution of household bleach containing 5.25%-6.15% sodium hypochlorite) until dry 

Surface Other

No Value Entered  

Surface More Than One

No Value Entered  

Microbes, OTCC, rDNA materials

10% bleach for a minimum of 20 minutes, Autoclave at a minimum of 121 Degrees C for a minimum of 45 minutes; MERI disposal

Microbes, OTCC, rDNA materials Other

No Value Entered  

Microbes, OTCC, rDNA materials More Than One

Any of the methods are used

Sharps

MERI disposal

Sharps Other

No Value Entered  

Sharps More Than One

No Value Entered  

Plasticware/glass

Autoclave at a minimum of 121 Degrees C for a minimum of 45 minutes; MERI disposal

Plasticware/glass Other

No Value Entered  

Plasticware Glass More Than One

Any of the methods are used 

BioToxins

 20% bleach for a minimum of 30 minutes  

BioToxins Other

No Value Entered 

Biological Toxins More Than One

No Value Entered  

Other

No Value Entered  

Other Material

No Value Entered  

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Autoclave Testing 

For information on how to test an autoclave and autoclave safety, please see our website: https://ehs.wisc.edu/labs-research/biological-safety/autoclaves/.

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Spill and Release Procedure

Biological spill guidance and spill procedure templates are available on the OBS websitehttps://ehs.wisc.edu/labs-research/biological-safety/biological-spill-protocols/.

If you would like to use these templates, complete the header (title) at the top of the page then create a customized protocol using the dropdowns provided within the template. In the header, the line above "Lab Biohazardous Spill Protocol for" should list the PI or laboratory name. The line below "Lab Biohazardous Spill Protocol for" should indicate when the procedure will be used (e.g., specify the biological materials, location(s), or other information that indicates when the spill protocol would be used). If the same procedure will be used for all locations, each room does not need to be listed in the header.

Guidance for completing the templates may be found at https://ehs.wisc.edu/labs-research/biological-safety/biological-spill-protocols/.


A transgenic release document example is available on the OBS website:
 Release of Transgenic Materials pdf

If you would like to use this template, please edit template as appropriate to specify procedures for biological materials handled in your laboratory (i.e., remove any release example(s) not applicable or include any additional procedures).

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Still have questions? Call the Office of Biological Safety (OBS) at 608-263-2037. We are happy to help.



Keywordsdisinfect, inactivate, disposal, autoclave testing, spill procedure   Doc ID43090
OwnerTara S.GroupARROW - Institutional Biosafety Committee
Created2014-08-21 15:22:08Updated2024-02-27 15:03:24
SitesARROW - Institutional Biosafety Committee
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