Bio-ARROW - SmartForm - Aerosol Containment

Aerosol Generating Activities

When choosing more than one containment option for an activity, explain when the different forms of containment are used in the “Containment More Than One” section.  For example, “activities with E. coli K12 are performed on a lab bench with no containment used; work with human cells is performed in a BSC.”

Activity	Activity Other	Containment	Containment Other	Containment More Than One
Surgery/tissue, organ, cell harvest/necropsy		Performed on lab bench, no containment used; BSC		Surgery, tissue, organ, cell harvest, and necropsies are performed in the BSC for mice administered virus.  Surgery
Blending, sonicating, homogenizing, of electroporation		Performed on lab bench, no containment used; BSC		RG1 microbes are handled on the lab bench.  All procdures with RG2 microbes, human materials, and toxins are performed in the BSC
Animal inoculations		BSC		RG1 microbes are handled on the lab bench.  All activities associated with RG2 microbes, human materials, and toxins are handle din the BSC.
Opening culture plates, ampoules, tubes		Performed on lab bench, no containment used; BSC		RG1 microbes are handled on the lab bench. All activities associated with RG2 microbes, human materials, and toxins are handled in the BSC.
Flaming inoculating needles, slides, or loops		Performed on lab bench, no containment used	Flaming is only performed with RG1 microbes, not in a BSC
Animal cage changes		Performed on lab bench, no containment used		Cage changes for mice that have been administered virus or human cells must be performed in the BSC. Cage changes for untreated transgenic mice may be conducted on the lab bench.
Vortexing		BSC; Performed on lab bench, closed containers		Vortexing is only performed with closed containers.
Pipetting		Performed on lab bench, no containment used; BSC		RG1 microbes are pipetted on the lab bench. All pipetting of RG2 microbes, human materials (cell culture/tissues), and toxins are performed in the BSC

Biological Materials Outside of Containment

If any activity in the “Aerosol generating activities” section is performed without containment on the benchtop, it should be addressed in this section. A brief description of the work being done and the agents worked with is sufficient. For instance, “All culturing and plasmid prep of E. coli K12 will be performed outside of containment.”

Flow Cytometry Containment

For work performed at the UWCCC flow cytometry laboratory, http://www.uwhealth.org/uw-carbone-cancer-center/for-researchers/shared-resources/flow-cytometry/flow-cytometry-shared-resource/27331, contact the staff to determine what type of containment is appropriate for the materials you are sorting.

Still have questions? Call the Office of Biological Safety (OBS) at 608-263-2037. We are happy to help.