Bio-ARROW - SmartForm - Aerosol Containment
Bio-ARROW help
Aerosol Generating Activities
When choosing more than one containment option for an activity, explain
when the different forms of containment are used in the “Containment
More Than One” section. For example, “activities with E.
coli K12 are performed on a lab bench with no containment used;
work with human cells is performed in a BSC.”
Activity
Activity Other
Containment
Containment Other
Containment More Than One
Surgery/tissue, organ, cell harvest/necropsy
Performed on lab bench, no containment used; BSC
Surgery, tissue, organ, cell harvest, and necropsies are performed in the BSC for mice administered virus. Surgery
Blending, sonicating, homogenizing, of electroporation
Performed on lab bench, no containment used; BSC
RG1 microbes are handled on the lab bench. All procdures with RG2 microbes, human materials, and toxins are performed in the BSC
Animal inoculations
BSC
RG1 microbes are handled on the lab bench. All activities associated with RG2 microbes, human materials, and toxins are handle din the BSC.
Opening culture plates, ampoules, tubes
Performed on lab bench, no containment used; BSC
RG1 microbes are handled on the lab bench. All activities associated with RG2 microbes, human materials, and toxins are handled in the BSC.
Flaming inoculating needles, slides, or loops
Performed on lab bench, no containment used
Flaming is only performed with RG1 microbes, not in a BSC
Animal cage changes
Performed on lab bench, no containment used
Cage changes for mice that have been administered virus or human cells must be performed in the BSC. Cage changes for untreated transgenic mice may be conducted on the lab bench.
Vortexing
BSC; Performed on lab bench, closed containers
Vortexing is only performed with closed containers.
Pipetting
Performed on lab bench, no containment used; BSC
RG1 microbes are pipetted on the lab bench. All pipetting of RG2 microbes, human materials (cell culture/tissues), and toxins are performed in the BSC
Biological Materials Outside of Containment
If any activity in the “Aerosol generating activities” section is performed without containment on the
benchtop, it should be addressed in this section. A brief description of the work being done and the agents worked with is sufficient. For instance,
“All culturing and plasmid prep of E. coli K12 will be performed outside of containment.”
Flow Cytometry Containment
For work performed at the UWCCC flow cytometry laboratory, http://www.uwhealth.org/uw-carbone-cancer-center/for-researchers/shared-resources/flow-cytometry/flow-cytometry-shared-resource/27331, contact the staff to determine what type of containment is appropriate for the materials you are sorting.
Still have questions? Call the Office of Biological Safety (OBS) at 608-263-2037. We are happy to help.