Freeze

This documentation pertains to the May 2025 Freeze of the CSF Roche Elecsys NTK - Gothenburg dataset.


Origin

These cerebrospinal fluid assay results come from the laboratory of Henrik Zetterberg and Kaj Blennow.

Sample Selection

Samples were included from observations at which at least one aliquot of CSF was available that had been stored in a Fisher 1.5 mL flip top tube.

For detailed information, refer to the WRAP and/or ADRC pre-analytic protocols for CSF and plasma collection, available on the Wisconsin Alzheimer's Program KnowledgeBase.

Sample and Participant Characteristics

This dataset includes 1040 observations on 642 participants (400 ADRC; 242 WRAP; 398 female; 32 URG; 51 MCI and 51 Dementia at first visit; 50 MCI and 52 Dementia at last visit; <5 participant(s) are aged 90+, of those not: mean first and last ages of 62.5 and 63.9, respectively; mean first and last days since baseline of 1228 and 1773, respectively). Per data sharing agreements with the Oneida nation, data from Native American participants are not included in this dataset.

Analytes

• Amyloid beta 40 (ABeta_1_40; pg/mL)
• Amyloid beta 42 (ABeta_1_42; pg/mL)
• Phosphorylated tau 181 (pTau; pg/mL)
• Total tau (tTau; pg/mL)
• Neurogranin (pg/mL)
• Neurofilament light-chain (NFL; pg/mL)
• Alpha synuclein (a_Synuclein; pg/mL)
• Glial fibrillary acidic protein (GFAP; ng/mL)
• sTREM2 (ng/mL)
• S100 (ng/mL)
• YKL40 (ng/mL)
• Interleukin-6 (IL_6; pg/mL)

Derived Variables

• AB42/AB40
• pTau181/AB42
• tTau/AB42
• 1/AB42
• pTau181 binary
• AB42/AB40 binary
• pTau181/AB42 binary

Detection Limits

• AB42: 200-1700 pg/mL
• pTau181: 8-120 pg/mL
• tTau: LLOQ 80 pg/mL
• IL-6: LLOQ 1.5 pg/mL

Methods

A first paper describing the use of these markers in the Wisconsin cohorts is here:

Van Hulle C, Jonaitis EM, Betthauser TJ, et al. An examination of a novel multipanel of CSF biomarkers in the Alzheimer’s disease clinical and pathological continuum. Alzheimers Dement. 2021;17(3):431-445. https://doi.org/doi:10.1002/alz.12204

Data Dictionary

Funding / Acknowledgments

Papers including data obtained from these assays should acknowledge NIH R01 AG027161 (the Wisconsin Registry for Alzheimer's Prevention) and/or NIH P30 AG062715 (the Wisconsin Alzheimer's Disease Research Center).

Papers with Wisconsin coauthors that include data from this set must submit abstracts, posters, talks and papers to Roche for pre-publication review.